B. Saha (Das), S. Sarkar* and
N. Paria1
Department of Botany, University
of Gauhati, Gopinath Bordoloi Nagar, Guwahati 781 014.
* Lecturer, Rangia
College, District- Kamrup (Assam).
1 Calcutta University,
Calcutta.
Seedling morphology of five important timber-yielding plants namely Gmelina arborea, Mimusops elengii, Polyalthia longifolia, Putranjiva roxburghii, and Terminalia arjuna have been studied. The taxonomic implications of seedling features are discussed.
In modern era, we are loosing many important plant species due to high pressure of urbanisation, industrialisation and deforestation. So the study of seedling morphology is very important to conserve these plants permanently and for easy identification. Several workers studied the seedling characters of different species of plants. Hill (1982) illustrated and discussed briefly the variations in cotyledon morphology in Malvastrum. Sampath Kumar (1982) stressed the use of cotyledon morphology at the generic, specific and varietal levels in the family. Kamilya and Paria (1993) studied on seedling morphology of some members of the Polygonaceae and its taxonomic implications. In this paper, the study of the morphology of seedlings of some commonly occurring timber-yielding plant species have been studied.
MATERIALS AND METHODS
In this present investigation, seedlings of different stages were collected
from different natural habitats in different places of Guwahati. They were
compared and identified with the help of seedlings raised from identified
seeds. At least ten specimens were studied from various localities. The
seedlings were dried and made into herbarium and preserved. The morphology
has been described following the terminology proposed by Burger (1972),
Hickey (1973) and Vogel (1980). For morphological diagnoses of seedlings,
the characters of cotyledons or paracotyledons, eophylls (first few leaves)
and hypocotyl were taken into consideration.
OBSERVATION
Gmelina arborea Roxb. Corom, pl.3, t-246.1818 & F1.Ind.
3:84.1832; C.B. Clarke in Hook-P. FBI. 4:581.1885; Kanjilal
et al.
FA 3; 466. 1939; Deb, FT 2:110.1983. Fam: Verbenaceae. Local name; Gomari
(Ass.) (Plate 1).

Germination epigeal, phanerocotylar. Taproot short, 1.8 - 2.9 cm. grayish white. Hypocotyl slightly curved, 1-1.3 cm. pale green, terete. Paracotyledons two, opposite, persistent up to 8-9 leaved stage, exstipulate, petiolar, Petiole + 1.3 cm. bases ensheathing node and some portion of internode forming a cup-like structure, tube + 1.9 cm. in length, greenish, blade fleshy, deep green, 1.8 -2.5 cm. obovate-oblong, apex bi-lobed, base cuneate, entire, primary veins 3 - parallely arranged, distinct, secondaries inconspicuous. Internode straight, long, terete, light green, length of first and second internodes are 6.8 & 1.8 cm respectively other shorter. First two leaves opposite, simple, petiolate, green, rhombioobovate, smooth, wavy margin, apex acuminate, base rounded, size of the first two leaves are 5.5 x 3.8cm & 4.5 x 4.3cm respectively. Primary vein one-distinct, secondary vein 4-5 pairs, opposite or alternate, exstipulate, small, green, subsequent leaves opposite, simple stipulate other characters same as that of first two leaves.
Mimusops elengii L.Sp.Pl.349. 1753; C.B. Clarke in Hook f.FBI.3.: 548. 1882; Kanjilal et al. FA 3: 197.1939 Deb, FT. 1:430. 1981 Fam: Sapotaceae. Local name: Bakul (Ass) (Plate 2).

Germination epigeal, phanerio cotylar. Tap root long, 5.5-7 cm creamy white. Hypocotyl slightly curved long 2.5-7.5 cm brown. Para-cotyledons two, opposite, persistent up to 7-8 leaved stage, exstipulate, blade smooth, green, rounded-sub-cordate, 2.5-2.8 cm apex and base rounded, dorsal surface smooth, ventral surface rough, entire, primary vein one, distinct, secondary vein 2-3 pairs opposite, distinct. Internodes short, 9-1.4 cm long, straight slightly curved green. First two leaves alternate, simple, oval-elliptic, glabrous, chartaceous, shining, green, entire, acuminate, acute at base, length of the 1st & 2nd leaves are 3.5 cm & 5.4 cm respectively. petiolate, petiole short, 2-7 cm primary vein one, distinct, other veins are invisible. Subsequent leaves alternate, simple green, large, other characters same as that of first two leaves.
Polyalthia longifolia (Sonn) Thw. Enum. 398, 1864, Hook.f & Thoms. in Hook.f FBI 1:62 1872, Kanjilal et al. FA 1(1):31 1934. Deb, FT. 1:87, 1981. Fam: Annonaceae, Local name : Debdaru (As) (Plate 3.)

Germination hypogeal, phanerocotylar. Tap root long, brown, Hypocotyl curved, 1.8-2.8 cm long, grayish brown. Paracotyledones two rudimentary,7-2.5 cm alternate or sub-opposite, lanceolate, petiolate acuminate, entire, petiole 1.5 cm greenish, acute at base, primary vein one, distinct, secondary veins invisible. Internodes long green, straight or slightly curved length of the 1st internode 2-4.5 cm 1st and 2nd internode 2.5-3.5 cm respectively, other shorter than the 1st and 2nd. First two leaves alternate, simple, short petiolate, laceolate, greenish, acuminate, entire or slightly wavy at margin, acute at the base, size of the first two leaves are 4.1 x 1.5cm and 5 x 1.9cm respectively, primary vein one, distinct, secondary veins 4.5 pairs alternate or sub-opposite, petiole 1.3cm subsequent leaves alternate, simple, stipulate, 2.3-3.5cm long other characters same as that of first two leaves.
Putranjiva roxburghii Wall. Tent. Fl. Nep. 61 and cat. 6814; Hook. f. FBI 5.336 1885. Kanjilal et al. FA. 4: 179.1940. Fam: Euphorbiaceae. Local name: Putranjiva (Ass.) (Plate 4).

Germination epigeal, Phanerocotylar. Tap root long, 4-5cm grayish or reddish white. Hypocotyl slightly curved, long, 5.5cm upper portion pale greenish, lower portion reddish, terete. Paracotyledons two, opposite, persistent up to 8-9 leaved stage, exstipulate; petiole + 3cm primary vein 3, parallely arrange, distinct, petiolar bases ensheathing node and small portion of internode forming a short cup like structure; cup 0.4-0.5cm in length greenish, blade smooth, green, cordate, 2.3-2.5cm apex rounded, base cordate-sub-cordate; dorsal surface smooth ventral surface rough. Internode short, 1.3-1.8cm terete green other shorter than the first. First two leaves alternate, simple, petiolate, green, ovate, lanceolate, serrate, apex & base acute; length of the first two leaves are 2.8 and 3.5 cm. respectively; primary vein distinct, other veins are invisible, stipulate or exstipulate, small, green subsequent leaves alternate, simple, other characters same as that of first two leaves.
Terminalia arjuna (Roxb.) wight & Arn. Prodr. 314. 1834; C.B. Clarke in Hook.f. FBI 2:447. 1878; Deb FT. 1:384. 1981. Fam : Combretaceae. Local name: Arjun (Ass) (Plate 5).

Germination epigeal, phanerocotylar. Tap root long, 1.5-6 cm. grayish. Hypocotyl curved, long 7-7.5cm lower portion yellowish white, upper portion light green. Paracotyledons two, large, opposite petiolate, persistent up to 8-9 leaved stage; petiole + 1.5cm petiolar bases ensheathing the internode to forma cup-like structure, cup + 5cm long; blade triangular, greenish, 2 x 3.5cm acute or rounded at apex, attenuated at base, entire, primary veins are, distinct, secondary veins 2-3 pairs, distinct. Internoded long, + 3cm greenish other internodes shorter, node swollen. Leaves clustered at the end of branches, first two leaves opposite, others alternate, simple, green, elliptic or elliptic-obovate, petiolate, coriaceous, puberulous, obtuse or apiculate at the apex, narrowed at the base, length of the first two leaves 2.5-3.5x7-9cm and length of the third leaf 4 x 1cm respectively, slightly wavy at margin; petiole + .5cm primary vein one, distinct; secondary veins 5-6 pairs, distinct. Subsequent leaves alternate simple, large, petiolate, other characters same as that of first two leaves.
DISCUSSION
Among the 5 plants, all species showed epigeal type of germination
except Polyalthia longifolia which shows hypogeal type of germination.
In Polyalthia longifolia, cotyledonary leaves are very rudimentary
in comparison to others. In Mimusops elengii, seedling growth is
very slow and it took 3 months where as Gmelina arborea showed very
quick seedling growth. It has completed all the stages within 7 days. Hypocotyl
is also very short. The area of cotyledonary leaves is large in Terminalia
arjuna.
CONCLUSIONS
The seedling features revealed in the present study show correlation
of juvenile characters with some adult characters. The data obtained in
the present study help in the demarcation of families. The above mentioned
five tree species of plants are very much important for timber and these
timbers are very useful and utilized in different ways and also have medicinal
value (Chopra, 1956; Kirtikar & Basu, 1975). With the help of seedling
morphology, we can identify these plants easily and escape them from deforestation.
LITERATURE CITED
Burger, H.D., 1972. Seedlings of some tropical trees and shrubs mainly
of South East Asia, Wageningen.
Chopra, R.N. et al. 1956, Glossary of Indian Medicinal Plants,
New Delhi.
Deb, D.B., 1981 & 1983. Flora of Tripura State. Vol I & II.
New Delhi.
Hickey, L.J., 1973. Classification of the architecture of dicotyledonary
leaves. Amer. J. Bot. 60. 17-33.
Hill, S.R., 1982. A monograph of the genus Malvastrum A Gray
(Malvaceae : Malveae). Rhodora 84 :1-409.
Hooker, J.D., 1872-1887. Flora of British India. Vol. 1-7 London.
Kamilya, P. and Paria, N.,1993. Seedling morphology of some member
of the Polygonaceae and its taxonomic implications. Rheedea.Vol.3(1): 29-34.
Kanjilal, U.N. et al. 1934-40. Flora of Assam. Vol 1-4. Shillong.
Kirtikar, K.R. and Basu, B.D., 1975. Indian Medicinal Plants (2nd
ed.in
4 Volumes).
Sampath Kumar, R., 1982. Studies on the cotyledonary leaves of some
Convolvulaceae. Taxon : 31:53-56.
Vogel, E.F. de., 1980. Seedling of Dicotyledons. Wageningen.